Table 2 Activation of wild-type and mutants dmIR by DILP2 and DILP5

From: Structure and activation of the Drosophila insulin receptor by three Drosophila insulin-like peptides

 

DILP5 (%)a

DILP2 (%)b

WT

100

100

Site-1

  

Q1040A

49.88 ± 6.204

62.34 ± 6.923

F1044A

47.81 ± 3.107

73.3 ± 6.97

R848A

30.54 ± 3.848

49.3 ± 2.803

R657A

77.82 ± 11.7

N.D.

Δ602–609

75.33 ± 4.766

N.D.

Site-2

  

V889R

82.12 ± 2.988

N.D.

S820R

71.04 ± 5.726

N.D.

FnIII-3−FnIII-3 interaction

  

D1251R

68.6 ± 6.589

54.09 ± 8.052

K1256A

70.01 ± 8.224

44.53 ± 7.604

  1. N.D. not determined.
  2. aLevels of dmIR autophosphorylation were normalized to total dmIR levels and are shown as intensities relative to that of dmIR-WT treated with 100 nM DILP5 for 10 min. Mean ± s.e.m.
  3. bLevels of dmIR autophosphorylation were normalized to total dmIR levels and are shown as intensities relative to that of dmIR-WT treated with 100 nM DILP2 for 10 min. Mean ± s.e.m.
Back to article page