Fig. 2: De novo assemblies for the C57BL/6 J (BL6J) and 129S1/SvImJ (129S1) reveal that the Chr4 KZFP gene cluster is much larger than previously estimated. | Nature Communications

Fig. 2: De novo assemblies for the C57BL/6 J (BL6J) and 129S1/SvImJ (129S1) reveal that the Chr4 KZFP gene cluster is much larger than previously estimated.

From: Young KRAB-zinc finger gene clusters are highly dynamic incubators of ERV-driven genetic heterogeneity in mice

Fig. 2: De novo assemblies for the C57BL/6 J (BL6J) and 129S1/SvImJ (129S1) reveal that the Chr4 KZFP gene cluster is much larger than previously estimated.The alternative text for this image may have been generated using AI.

a Scheme of the experimental setup and analysis to achieve the BL6J and 129S1 genome assemblies and curation of new KZFP gene annotation. b, c BUSCO statistics of BL6J (b) and 129S1 (c) de novo assemblies, after removing unplaced and redundant contigs. d Comparison of the Chr4 KZFP gene cluster (defined as 1 bp downstream of the Tnfrsf8 gene and 1 bp upstream of the Miip gene) in de novo BL6J assembly with the reference GRCm39 assembly. Annotated genes are indicated as arrow heads. Gaps in the GRCm39 assembly are indicated in black. The arc plot on top of the de novo BL6J assembly indicates regions of self-identical sequence within the locus. e Comparison of the Chr4 KZFP gene cluster (defined as 1 bp downstream of the Tnfrsf8 gene and 1 bp upstream of the Miip gene) in de novo 129S1 assembly with the available 129S1/SvImJ_v3 (GCA_921998555.2) assembly. Annotated genes are indicated as arrow heads; for 129S1.v3 assembly, genes from the Gencode M32 annotation lift-over are shown. Gaps in the 129S1.v3 assembly are indicated in black. The arc plot on top of the de novo 129S1 assembly indicates regions of self-identical sequence within the locus.

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