Fig. 5: Impact of polypeptide length on phase separation behavior.
From: Automated navigation of condensate phase behavior with active machine learning
This figure displays nine phase diagrams illustrating the automated mapping of phase separation for combinations of poly-L-(lysine) and poly-L-(aspartic acid) with varying chain lengths. Panels represent phase diagrams for poly-L-(lysine) with a chain length of 20, combined with poly-L-(aspartic acid) of lengths 30 (A), 100 (B), and 200 (C). Panels show poly-L-(lysine) with a chain length of 100, paired with poly-L-(aspartic acid) lengths of 30 (D), 100 (E), and 200 (F). Panels (G-I) depict poly-L-(lysine) with a chain length of 250, combined with poly-L-(aspartic acid) lengths of 30 (G), 100 (H), and 200 (I). Datapoints are marked as dots, with blue indicating phase separation and red indicating no phase separation. Each phase map includes a background color gradient derived from predictions based on 72 datapoints per combination, acquired over nine cycles of eight datapoints. Remaining cycles and entropy maps are reported in Supplementary Figs. 27–42. Phase boundaries are indicated by dotted lines; in some cases, these may be partially obscured by overlapping contour lines. Total polypeptide consumption per phase diagram: 4.0–4.9 mg poly-L-(aspartic acid) and 3.9–5.4 mg poly-L-(lysine).
