Fig. 3: Developmental trajectory of SST-IN visual response amplitude and selectivity.

a Responses of example SST-INs (blue) and PNs (black) to drifting grating stimuli of varying sizes at P15 and P28. Vertical dashed lines indicate visual stimulus onset. Shaded areas indicate mean ± SEM. b Population average visual responses of the subset of visually responsive SST-INs (upper, blue) and PNs (lower, black) to stimuli of varying size at each age. Responses are Z-scored to the 1-s baseline period before the stimulus onset for periods of quiescence (Q, light colors) and locomotion (L, dark colors). c Cumulative probability distribution of response amplitude at the preferred stimulus size for visually responsive SST-INs in each age group from (b) (left; P15–17: n = 41 cells, 5 mice; P18–20: n = 80 cells, 7 mice; P21–23: n = 95 cells, 9 mice; P24–26: n = 141 cells, 10 mice; P27–29: n = 77 cells, 6 mice) and PNs (right; P15–17: n = 268 cells, 6 mice; P18–20: n = 217 cells, 7 mice; P21–23: n = 201 cells, 6 mice; P24–26: n = 397 cells, 6 mice; P27–29: n = 225 cells, 6 mice). d Boxplots of response amplitudes at the preferred stimulus size for each age group from (c) for SST-INs (blue) and PNs (black). The central mark indicates the median, box boundaries indicate the 25th and 75th percentiles, and whiskers indicate the range of the data. e Boxplots of locomotion-mediated gain modulation of visual response amplitudes in SST-INs (blue) and PNs (black) across age groups from (c). *p < 0.05, **p < 0.01, ***p < 0.001, all statistics calculated with a linear mixed-effects regression model with age as a fixed effect and mouse as a random effect.