Fig. 1: iNap1 detected increased cytosolic but not mitochondrial NADPH levels in senescent ECs.
From: Elevating cytosolic NADPH metabolism in endothelial cells ameliorates vascular aging
Representative images of the fluorescence ratio of cyto-iNap1 and cyto-iNapc (a), mito-iNap3 and mito-iNapc (b) in HAECs upon diamide treatment. Scale bar: 10 μm. Response of the fluorescence ratio of cyto-iNap1 and cyto-iNapc (c), mito-iNap3 and mito-iNapc (d) to various NADPH concentrations in permeabilized HAECs. n = 3 biologically independent samples. Time-dependent changes in cyto-iNap1 (e) and mito-iNap3 (f) in response to acute addition of diamide. n = 3 biologically independent samples. The fluorescence ratio of cyto-iNap1 and cyto-iNapc, mito-iNap3 and mito-iNapc with or without Ang II (g, h), Glu (i, j), ET-1 (k, l), Hcy (m–n) and replicative passage (o, p) treatment. n = 3 biologically independent samples, 106, 112, 101 and 101 cells (g), 110, 99, 107 and 105 cells (h), 122, 112, 118 and 126 cells (i), 109, 115, 117 and 117 cells (j), 104, 104, 107 and 105 cells (k), 102, 104, 114 and 116 cells (l), 105, 108, 105 and 106 cells (m), 106, 105, 106 and 104 cells (n), 122, 122, 122 and 105 cells (o), 112, 118, 120 and 103 cells (p). The fluorescence ratio of cyto-iNap1 and cyto-iNapc (q), mito-iNap3 and mito-iNapc (r) in young and old ECs. n = 3 biologically independent samples, 125, 108, 110, and 118 cells (q) and 104, 115, 122 and 122 cells (r). s Relative NADPH content in thoracic aorta from young and old mice. n = 6 mice. t GO analyses of microarray data of old vs young HAECs. Data are presented as mean values ± SEM. Statistical significance was assessed by Two-way ANOVA followed by Å Ãdák’s multiple comparisons test (c–f), One-way ANOVA followed by Tukey’s multiple comparisons test (g–r), two-tailed unpaired Student t test (s).
