Fig. 4: NADPH was required for G6PD to protect against EC senescence.
From: Elevating cytosolic NADPH metabolism in endothelial cells ameliorates vascular aging
a The fluorescence ratio of cyto-iNap1 and cyto-iNapc with or without Ang II, TpNOX and G6PD overexpression in HAECs. n = 3 biologically independent samples, 116, 107, 112, 109, 119, 115, 111, 121, 106 and 155 cells for respective groups. Scale bar: 10 μm. b G6PD, p16 and p21 protein levels in HAECs with or without Ang II, TpNOX and G6PD overexpression. c RT-PCR analysis of IL-1β, IL-6, IL-8 and CXCL1 mRNA levels in HAECs with or without Ang II, TpNOX and G6PD overexpression. n = 4 biologically independent samples. Representative SA-β-Gal (d) and EdU fluorescence (e) staining images of HAECs with or without Ang II, TpNOX and G6PD overexpression. Scale bar: 50 μm. f The fluorescence ratio of cyto-roGFP with or without Ang II, G6PD and TpNOX overexpression in HAECs. n = 3 biologically independent samples, 100, 106, 104, and 90 cells for respective groups. Scale bar: 10 μm. g HDAC3 protein levels in HAECs with or without pronase and increase amount of NADPH incubation. h HDAC3 activity in HAECs with increase amount of NADPH incubation. n = 4 biologically independent samples. i HDAC3 activity with or without Ang II, G6PD and TpNOX overexpression in HAECs. n = 4 biologically independent samples. j Ac-H3, Ac-H4, HDAC3, Ac-NF-κB, IL-1β and IL-6 protein levels with or without Ang II, G6PD and TpNOX overexpression in HAECs. Data are presented as mean values ± SEM. Statistical significance was assessed by One-way ANOVA followed by Tukey’s multiple comparisons test (a, f, h, i), Two-way ANOVA followed by Tukey’s multiple comparisons test (c).
