Fig. 5: Blocking SUMOylation leads to disruption of the synovial sarcoma signature and to induction of mesenchymal differentiation.

A Normalized enrichment scores after KEGG analysis of RNA-seq data demonstrating pathways altered by 100 nM TAK-981 treatment for 36 h in the indicated SS cells. B Venn diagram of genes significantly downregulated following SS18::SSX knockdown or treatment with 100 nM TAK-981 for 36 h. The significance of the gene set overlaps (p value) was calculated with Chi-square test under the assumption of 17,611 expressed transcripts. C Venn diagram of genes significantly upregulated following SS18::SSX knockdown or treatment with 100 nM TAK-981 for 36 h. The significance of the gene set overlaps (p value) was calculated with Chi-square test under the assumption of 17,611 expressed transcripts. D Volcano plot of the most down- or upregulated 1000 genes following TAK-981 treatment (detected by RNA-seq), plotted with the commonly downregulated (light orange dots) or the commonly upregulated genes (dark orange dots) after SS18::SSX KD, among the indicated SS cell line. The p values were adjusted using a False Discovery Rate (FDR) multiple testing correction method.