Table 1 Summary of KD (dimer dissociation constant = dimer-monomer equilibrium constant), koff (dimer dissociation rate constant), and kon (dimer formation rate constant) for three ORs without agonists and dimer reference (CD28) and monomer reference (TMLDLR) molecules at 37 °C as well as MOR at 20 °C and FPR(D71A) and D2R dopamine receptor at 37 °C41

From: Single-molecule methods for characterizing receptor dimers reveal metastable opioid receptor homodimers that induce functional modulation

ORs

KD (copies/µm2)

koff (s–1)

kon (µm2/copies/s)

KOR

6.04 ± 0.57

6.71 ± 1.17

1.11 ± 0.22

MOR

15.47 ± 1.54

8.47 ± 0.86

0.55 ± 0.08

DOR

16.62 ± 0.53

8.00 ± 0.96

0.48 ± 0.06

CD28

0.0093 ± 0.03

N.D.

N.D.

TMLDLR

132.00 ± 21.00

N.D.

N.D.

MOR (20 °C)28

27.43 ± 11.80

0.56 ± 0.21

0.02 ± 0.004

KOR (20 °C)

5.69 ± 01.11

4.63 ± 0.11

0.81 ± 0.16

KOR (perhaps 20 °C)35

32.00 ± 15.00

  

FPR(D71A)

2.77 ± 0.55

9.26 ± 0.17

3.61 ± 0.72

FPR(D71A)41

3.60 ±0.58

11.0 ± 1.90

3.06 ± 0.72

D2R

33.00 ± 4.61

7.5 ± 0.23

0.23 ± 0.03

D2R40

 

14.6 ± 1.03

 
  1. These thermodynamic and kinetic constants have rarely been measured, and all of those we found in the literature28,35,41 are also listed in this table for comparison.
  2. N.D. for no data.
  3. SEM variations are somewhat large, probably due to limited number of observed movies (approximately 20 movies for 20 cells; the exact numbers are in Supplementary Data 2, along with all other statistical parameters). For comparison with the KD, koff, and kon values for membrane molecules found in the literature (only four cases were found and shown here), we also performed the measurements for MOR at 20 °C28, D2R dopamine receptor (D2R)40, and formyl peptide receptor (FPR(D71A)), at 37 °C41.
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