Fig. 6: FM4-64 imaging reveals vesicle release deficit associated with the LINC02449 alternative allele and CPLX1 dependence. | Nature Communications

Fig. 6: FM4-64 imaging reveals vesicle release deficit associated with the LINC02449 alternative allele and CPLX1 dependence.

From: Gain of Alternative Allele Expression of LINC02449 at rs149707223 in Schizophrenia and Bipolar Disorder: Inducing Synaptic Transmission and Behavioral Deficits in Mice

Fig. 6: FM4-64 imaging reveals vesicle release deficit associated with the LINC02449 alternative allele and CPLX1 dependence.The alternative text for this image may have been generated using AI.

A, B FM4-64 fluorescence decay following KCl-induced depolarization in SH-SY5Y cells (A) overexpressing control vector (Ctrl; n = 8 cells), LINC02449 reference allele (Ref; n = 5 cells), or LINC02449 alternative allele (Alt; n = 7 cells), and in SK-N-SH cells (B; Ctrl: n = 5; Ref: n = 7; Alt: n = 3) for replication. C Rescue experiment in SK-N-SH cells showing that knockdown of CPLX1 (shCPLX1) attenuates the enhanced vesicle release phenotype induced by the LINC02449 Alt allele. Ref group was co-transfected with control shRNA (Ref+shCtrl; n = 10), while the Alt group was co-transfected with either shCtrl (Alt + shCtrl; n = 14) or shCPLX1(Alt + shCPLX1; n = 11). Data are means ± SEMs. P-values were calculated using the Wilcoxon rank-sum and signed-rank tests. Source data are provided as a Source Data file.

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