Fig. 6: Manipulation of CRISPR/Cas9 genome editing by MAAD bioorthogonal reaction.
From: A catalyst-free bioorthogonal reaction for malononitrile addition to azodicarboxylates
a Quantification of CRISPR/Cas9 caused indels of target loci in cells using M11-modified sg-SLX4IP under the indicated conditions. A9 (16 μM) was added to the cells 4 h after sgRNA transfection. b Quantification of CRISPR/Cas9 caused indels of target loci in cells using M11-modified sg-SLX4IP under the indicated conditions. A9 (16 μM) was added to the cells 1 h after sgRNA transfection. c Comparing the efficiency of the Cas9 small molecule Brd0539 and the MAAD bioorthogonal reaction to silencing of the native/M11-modified sgRNA guided CRISPR/Cas9 genome editing system. Error bars represent means ± SD (n = 3 biological replicates), significance was determined by two-tailed unpaired Student’s t-test (ns, not significant; ***P < 0.001, ****P < 0.0001). d The ratio of ON-target indels to 2MM OFF-target indels detected in each indicated condition. e The ratio of ON-target indels to total OFF-target indels detected in each indicated condition. f GUIDE-seq results of native sg-HEKs4 or M11-modified sg-HEKs4 guided genome editing under the indicated conditions. Source data are provided as a Source Data file.
