Fig. 3: Investigation of the 1:2 complexation behavior between R-C[4]B and ThT in 10 mM PBS buffer solution. | Nature Communications

Fig. 3: Investigation of the 1:2 complexation behavior between R-C[4]B and ThT in 10 mM PBS buffer solution.

From: Chiral macrocycles forming high-affinity host-guest homoternary complexes for chiral discrimination and amplified circularly polarized luminescence

Fig. 3: Investigation of the 1:2 complexation behavior between R-C[4]B and ThT in 10 mM PBS buffer solution.The alternative text for this image may have been generated using AI.

a Schematic illustration of R-C[4]B@2ThT homoternary complex. b Isothermal titration calorimetry (ITC) isotherms obtained by adding ThT (800 μM) into a 19.5 μM solution of R-C[4]B at 25 °C. c The analyzed data of ITC isotherms with the sequential binding model. d UV-Vis titration spectra obtained by adding R-C[4]B (up to 18 μM) into a 10.0 μM solution of ThT. e The fitting curve plot by tracking absorbance at λ = 430 nm. f Fluorometric titration spectra obtained by adding R-C[4]B (up to 3.6 μM) into a 2.0 μM solution of ThT. g The fitting curve plot by tracking emission intensity at λ = 600 nm, with the nonlinear curve fitting by using 1:2 host-guest binding model. The binding constant is the average of two experiments and the error indicated is the calculated standard deviation.

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