Table 1 Binding affinities of R-C[4]B towards selected analytes determined by fluorometric (FL) titrations, UV-Vis (UV) titrations, and/or ITC titrations in PBS buffer solution, and schematic illustration of thermodynamic parameters
Guests | K1 (M−1)a | K2 (M−1)b | K12 (M−2) | logα | Method |
|---|---|---|---|---|---|
G1 | (2.4 ± 0.9) × 106 | (1.5 ± 0.9) × 103 | 3.6 × 109 | –2.6 | FL |
G2 | (6.3 ± 0.1) × 105 | (3.5 ± 1.1) × 105 | 2.2 × 1011 | 0.35 | ITC |
G3 | (4.2 ± 0.8) × 105 | (5.7 ± 1.5) × 104 | 2.4 × 1010 | –0.27 | ITC |
G4 | (2.1 ± 0.1) × 106 | (1.6 ± 0.6) × 105 | 3.4 × 1011 | –0.52 | ITC |
G5 | (2.1 ± 0.2) × 106 | (2.9 ± 0.9) × 105 | 6.1 × 1011 | –0.26 | ITC |
G6 | (3.2 ± 2.5) × 106 | (1.6 ± 0) × 106 | 5.1 × 1012 | 0.3 | ITC |
G7 | (6.2 ± 1.3) × 106 | (1.6 ± 0) × 107 | 9.9 × 1013 | 1.0 | FL |
(1.4 ± 0.2) × 107 | (1.5 ± 0.5) × 106 | 2.1 × 1013 | –0.37 | ITC | |
G8 | (2.7 ± 0.8) × 107 | (6.8 ± 0.8) × 106 | 1.8 × 1014 | 0.0032 | ITC |
ThT | (9.3 ± 0.4) × 108 | (3.0 ± 0.7) × 106 | 2.8 × 1015 | –1.9 | FL |
(1.5 ± 0.7) × 109 | (2.8 ± 0.8) × 106 | 4.2 × 1015 | –2.1 | UV | |
(5.4 ± 1.5) × 108 | (2.4 ± 0.6) × 106 | 1.3 × 1015 | –1.8 | ITC | |
MB | (8.8 ± 1.4) × 109 | (5.6 ± 0.8) × 106 | 4.9 × 1016 | –2.6 | UV |
(2.1 ± 1.1) × 109 | (5.0 ± 0.9) × 106 | 1.0 × 1016 | –2.0 | ITC | |
TO | (9.2 ± 1.6) × 109 | (2.6 ± 0.6) × 108 | 2.4 × 1018 | –0.95 | FL |
(3.4 ± 1.7) × 109 | (4.9 ± 0.5) × 107 | 1.7 × 1017 | –1.2 | UV | |
(1.9 ± 0.1) × 109 | (4.4 ± 2.1) × 107 | 8.4 × 1016 | –1.0 | ITC | |
Thermodynamic parameters[b]
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