Fig. 2: A proportion of parasites in the cattle bloodstream have higher transcript levels of pyruvate metabolism linked genes. | Nature Communications

Fig. 2: A proportion of parasites in the cattle bloodstream have higher transcript levels of pyruvate metabolism linked genes.

From: Trypanosoma brucei cattle infections contain cryptic transmission-adapted bloodstream forms at low parasitaemia

Fig. 2

A Biological process GO terms enriched for cluster 3 marker genes. Plot indicated the fold enrichment (x-axis), number of genes for each term (size) and the adjusted p-value (colour scheme) for each term (Benjamini-Hochberg adjusted p-values from Fisher’s exact test). B Dot plot of the average expression of selected cluster 3 markers genes linked to terms “pyruvate metabolic process” and the “TCA cycle”. C Log2 fold change (Cluster 3 compared to clusters 0, 1 and 2 combined) was calculated for each gene. Dashed lines represent transport processes. Grey circles indicate genes were not significantly different in cluster 3 relative to other clusters. Genes: 1, hexose transporters; 2, hexokinase, Tb927.10.2010 and Tb11.v5.0732; 3, glucose-6-phosphate isomerase; 4, phosphofructokinase, Tb927.3.3270; 5, fructose-1,6-bisphosphatase, Tb927.9.8720; 6, aldolase; 7, triosephosphate isomerase; 8, glycerol-3-phosphate dehydrogenase; 9, glycerol kinase, Tb927.9.12570; 10, glyceraldehyde 3-phosphate dehydrogenase, Tb927.6.4280 and Tb927.6.4300; 11, phosphoglycerate kinase, Tb927.1.710; 12, phosphoglycerate mutase; 13, enolase; 14, pyruvate kinase 1, Tb927.10.14140 and Tb11.v5.0605; 15, alanine aminotransferase, Tb927.1.3950; 16, pyruvate phosphate dikinase, Tb927.11.6280; 17, Phosphoenolpyruvate carboxykinase, Tb927.2.4210; 18, glycosomal malate dehydrogenase, Tb927.10.15410; 19, glycosomal fumarate hydratase; 20, glycosomal NADH-dependent fumarate reductase, Tb927.5.930; 21, glucose-6-phosphate dehydrogenase, Tb927.10.2490; 22, 6-phosphogluconolactonase, Tb927.11.6330; 23, 6-phosphogluconate dehydrogenase; 24, ribulose-5-phosphate epimerase; 25, ribose 5-phosphate isomerase; 26, transketolase, Tb927.8.6170; 27, transaldolase, Tb927.8.5600; 28, ribokinase, Tb927.11.1020; 29, malic enzyme, Tb927.11.5450; 30, Mitochondrial pyruvate carrier 2; 31, FAD-dependent glycerol-3-phosphate dehydrogenase; 32, NADH dehydrogenase (NDH2), Tb927.10.9440; 33, Alternative oxidase, Tb927.10.7090; 34, mitochondrial fumarate hydratase, Tb927.11.5050; 35, mitochondrial NADH-dependent fumarate reductase, Tb927.10.3650; 36, mitochondrial malate dehydrogenase, Tb927.10.2560; 37, citrate synthase; 38, aconitase,Tb927.10.14000; 39, isocitrate dehydrogenase, Tb927.8.3690; 40, 2-oxoglutarate dehydrogenase E1 component, Tb927.11.9980; 41, 2-oxoglutarate dehydrogenase E1 component, Tb927.11.1450; 42, 2-oxoglutarate dehydrogenase E2 component, Tb927.11.11680; 43, succinyl-CoA synthetase α, Tb927.3.2230; 44, succinyl-CoA ligase β, Tb927.3.2230; 45, glutamine synthetase; 46, glutamate dehydrogenase, Tb927.9.5900; 47, pyruvate dehydrogenase E1 α subunit, Tb927.10.12700; 48, pyruvate dehydrogenase E1 β subunit, Tb927.3.1790; 49, dihydrolipoamide acetyltransferase; 50, pyruvate dehydrogenase complex E3; 51, L-threonine 3-dehydrogenase, Tb927.6.2790; 52, 2-amino-3-ketobutyrate coenzyme A ligase, Tb927.8.6060; 53, Acetyl-CoA hydrolase (ACH), Tb927.3.4260; 54, Succinyl-CoA:3-ketoacid coenzyme A transferase (ASCT), Tb927.8.6060. Source data are provided as a Source data file.

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