Fig. 7: PEC cell confirmation by cross-species integration and RNAscope analysis.
From: A multimodal cross-species comparison of pancreas development
a UMAP of sysVI-integrated human and pig scRNA-seq pancreas atlases. The black circle indicates cells co-clustering with the pig PEC cluster. b UMAPs of split views of human (left) and pig (right) data overlayed with the sysVI-integrated embedding, and conserved PEC marker gene (ASPM and ECT2) expression in each species. c UMAP of sysVI-integrated mouse and pig scRNA-seq pancreas atlases. The black circle indicates cells co-clustering with the pig PEC cluster. d UMAPs of split views of mouse (left) and pig (right) data overlayed with the sysVI-integrated embedding, and conserved PEC marker gene (ASPM and ECT2) expression in each species. e Detection of NKX2-2⁺ cells co-expressing ASPM (left) or ECT2 (right) in wild-type pig pancreas samples (E30 and E64) by RNAscope assay. Yellow arrows indicate double-positive cells. Scale bar 10 µm. Images are representative of 3 pig pancreas samples per time point. a–d: scRNA-seq of pancreatic cells from wild-type and INS-eGFP pigs. Detailed sample information is provided in Supplementary Data 1.
