Fig. 1: An example of raw images and time courses from the DNA-PAINT experiments used in this study.
From: Discriminating single-molecule binding events from diffraction-limited fluorescence
a Schematic of the two DNA-binding domains used to collect diffraction-limited fluorescent spots (adapted from ref. 11). Both domains share the same dye ("ATO532'') and strand ("10nt P3''), but differ in their complementary sequences: Domain 1 uses “8nt P3'” (a partial complement of P3), while Domain 2 uses the fully complementary “10nt P3'''. Example frames from the raw video data are shown next to the corresponding domains. b Example time courses annotated with diffraction-limited fluorescent spots from binding events in Domain 1 and Domain 2. Domain 2 exhibits longer binding durations than Domain 1 (dwell time analysis shows ton as 2.30 ± 2.38 s for Domain 1 and 11.56 ± 16.27 s for Domain 2, with survival curves provided in Supplementary Fig. 1), while both show similar intensity jumps. The length-based method classifies molecules by total binding durations, whereas the image-based method classifies individual events. Axis offsets (+17133/+18117, +6229/+271) indicate base intensity and event start frame, respectively. Source data are provided as a Source Data file.
