Fig. 7: Differential protein enrichment or depletion in uninfected and P. berghei- or P. falciparum-infected saliva.

Volcano Plot of differential protein expression between uninfected (U) and P. berghei-infected saliva (Pb) (a) and uninfected and P. falciparum-infected saliva (b). Differential expression was tested with a two-sample Student’s t-test with permutation-based FDR control (q ≤ 0.05) using a SAM-style variance parameter (s₀ = 0.1). The x-axis represents log₂ fold changes, while the y-axis shows significance (–log₁₀ p-value). Proteins depleted in infected saliva are marked in blue, and proteins enriched are marked in red. Only proteins detected in both conditions are included. Heatmap of differentially expressed proteins between uninfected and P. berghei-infected (c), and uninfected and P. falciparum-infected saliva (d). Protein expression values were Z-transformed and normalized across conditions. Rows (proteins) are ordered by function and clustered using Euclidean distance. Dot plot showing the abundance of classical saliva proteins in uninfected and P. berghei-infected (e), and uninfected and P. falciparum-infected mosquitoes (f). Each dot represents Log₂ LFQ values for individual proteins under uninfected (gray) and infected (blue) conditions. Proteins are listed on the vertical axis, and Log₂ LFQ values on the horizontal axis indicate relative abundance. The shadowed areas indicate abundant proteins differentially expressed. Source data are provided as a Source Data file.