Fig. 8: Histological immunofluorescence and immuno-transmission electron microscopy of P. berghei-iSGs. | Nature Communications

Fig. 8: Histological immunofluorescence and immuno-transmission electron microscopy of P. berghei-iSGs.

From: High-resolution proteomics unveils salivary gland disruption and saliva-hemolymph protein exchange in Plasmodium-infected mosquitoes

Fig. 8

These images display the distribution and association of immune proteins with the surface of P. berghei parasites in the SGs. Staining includes TEP15, PPO6, transferrin (green), circumsporozoite (CS, red), and WGA (magenta). Insert provides a closer view of stained P. berghei parasites, highlighting the spatial relationship between immune proteins and the parasite surface. Bottom images: immuno-TEM images showing the detailed interaction between immune proteins and P. berghei parasites — arrowheads showing immunogold staining of TEP15, PPO6, and transferrin, emphasizing the close association of immune proteins with the parasite surface (n = 3 independent experiments for immunofluorescence, n = 1 transmission electron microscopy).

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