Table 1 Evidence of SNAC/semaglutide co-aggregation in water based on convection-corrected 1H DOSY NMR

From: Permeation enhancer-induced membrane defects assist the oral absorption of peptide drugs

Sample

D (m2 sec–1)

R (nm)

100 mM SNAC

3.38 × 10−10

0.74

2.5 mM semaglutide

8.34 × 10−11

3.41

100 mM SNAC +

2.5 mM semaglutide

SNAC: 2.79 × 10−10

SNAC:a 2.44 × 10−10

Semaglutide:a 6.17 × 10−11

0.83

0.94

3.72

  1. The table lists the diffusion coefficients (D) and corresponding hydrodynamic radii (R) of SNAC and semaglutide, calculated with the Stokes-Einstein Equation. The diffusion coefficient of the solvent was used to correct for slight variations in sample viscosity.
  2. aWe hypothesize that free SNAC and SNAC in the cluster around semaglutide exchange rapidly on the NMR timescale, which leads to distinct diffusion bands for SNAC and semaglutide resonances.
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