Fig. 5: MIAA exerts anti-obesity effects by upregulating Slc2a4.
a Venn plots showing the results of KEGG enrichment analysis from three experiments (n = 4 per group). b Venn plots showing the results of differential gene analysis of insulin resistance from three experiments (n = 4 per group). c The mRNA expression level of Slc2a4 in liver of mice after 8 weeks of dietary interventions. d The mRNA expression level of Slc2a4 in liver of mice after 12 weeks of exposure to HFD, HFD + LRD80% and HFD + FMTLRD80%. e The mRNA expression level of Slc2a4 in liver of mice after 12 weeks of exposure to HFD + PBS and HFD + P. goldsteinii. f The mRNA expression levels of Slc2a4 in liver of mice after 12 weeks of exposure to HFD + NS and HFD + MIAA. g The expression levels of SLC2A4 mRNA in the cells after treated with MIAA for 24 hours in the insulin-resistant THLE-2 cell model. h Flow chart of the animal experiments. i, j The body weight and body weight gain of mice in each group over the course of the 12-week experiment. k–n Glucose metabolism of mice in each group at the end of experiment including GTT with AUC and ITT with AUC. o The weights of liver and eWAT of mice. Each circle represents an independent experiment (a). Each point in graphs (c–f, j, l, n, o) represents the value obtained in an independent mouse(n = 6 per group). Data were presented as mean ± S.E.M. P values were calculated with the one-way ANOVA followed by Tukey’s post-hoc analysis (c, d, g), two-tailed Mann-Whitney test (e), unpaired two-tailed Student’s t test (f, j, l, n, o), two-way ANOVA repeated-measure with Sidak’s multiple comparisons test (i, k, m) and exact P value were shown in figure panels. Source data are provided in a Source data file.
