Fig. 3: Functional coupling of the PUL, ANT, and VIM/VOP nuclei with SOZ during seizures.

a Representative examples of thalamocortical connectivity profiles of PUL (right, S03), ANT (middle, S23), and VIM/VOP (left, S25) with matched SOZ. For all nuclei, the top panel shows the cortical recording in the contacts of the SOZ, the middle panel shows the thalamic recording, and the bottom panel shows the h2 correlation coefficient. The gray shaded area includes the seizure duration (from onset to termination). b Boxplot of the mean h2 correlation for each seizure in matched and unmatched thalami, compared to mean h2 correlation during a baseline epoch preceding the seizure. Three representative patients are shown (right: S01, matched PUL, unmatched ANT; middle: S21, matched ANT, unmatched PUL; left: S12, matched PUL, unmatched VIM/VOP). c Raw traces of h2 correlation coefficient (smoothed for visualization) for a representative seizure for each patient of (b). The gray shaded area includes the seizure duration (from onset to termination). d Schematic illustrating the hodologically matching (bold colored lines) and unmatching (dashed gray lines) thalamocortical connections, created as detailed for Fig. 1a. e Violin plot showing the mean h2 coefficient across seizures for each matched (top) and unmatched (bottom) thalamus-SOZ pair. f Box plot of mean h2 coefficient across seizures during initiation, middle, and termination phase of each seizure. Each dot represents a patient. g Percentage difference of the change in h2 coefficient during seizures between matched and unmatched nuclei, across different frequency bands of interest. For all boxplots, the whiskers extend to the maximum spread not considering outliers, central, top, and bottom lines represent median, 25th, and 75th percentile, respectively. In all violin plot, the center line indicates the median, box bounds represent the first and third quartiles, whiskers extend to the most extreme data points within 1.5× the interquartile range, and points outside this range are plotted as outliers. For all panels, statistical significance and rejection of the null hypothesis of no difference of means with a 95% CI, was assessed with two-tail bootstrapping with Bonferroni correction. Triple asterisks indicate p << 0.0001.