Fig. 6: Transcriptional profile and microbial population changes with viral inoculation in contaminated soils.
a Expression of genes in samples inoculated with active viruses compared to controls. Statistical significance for data analysis was assessed using a two-sided t-test (n = 5). Gene abundance is significantly up-regulated or down-regulated with a log2-fold (fc) variation. When log2 (fc) >0, the gene in the sample is significantly upregulated compared to the control, and vice versa. Each circle represents a gene, purple for up-regulated, blue for down-regulated, and yellow for C fixation functional genes. b Temporal changes in gene expression of C fixation genes. c Variation in the relative abundance of the major groups of autotrophic bacteria and C fixation capacity of MAGs. The area map shows the change in the relative abundance of autotrophic bacteria after viral inoculation. In the pie chart, dark green shows that MAGs with CO2 fixed genes, light green shows that MAGs with genes except CO2 fixed genes in the carbon fixation pathway, and gray shows that MAGs with no C-fixation related genes.
