Fig. 4: GroES can be accommodated on RNCs bound by GroEL.
From: GroEL/ES chaperonin unfolds then encapsulates a nascent protein on the ribosome
a GroEL/ES:RNC co-sedimentation assay. Two different orders of addition were followed. Left: RNCs were incubated with GroEL/ES before addition of ATP/BeFx. Right: GroEL/ES was pre-mixed with ATP/BeFx before incubation with RNCs. b GroES co-sediments with a GroEL:RNC complex. Immunoblot of the resuspended ribosomal pellets from triplicate co-sedimentation assays described in (a), probed using antibodies against GroEL, GroES and the ribosomal protein S2. c Stoichiometry of GroEL:RNC complexes. Mean intensity-based absolute quantification (iBAQ) values for GroEL in resuspended ribosomal pellets from co-sedimentation assays described in (a). Values are normalised to the average iBAQ of all ribosomal proteins in each sample. Data are presented as mean ± SD, n = 3 independent co-sedimentation assays. Source data are provided as a Source Data file and in Supplementary Data 2.
