Fig. 5: Nascent chains occupy the central cavity of GroEL/ES.
From: GroEL/ES chaperonin unfolds then encapsulates a nascent protein on the ribosome
a Map of crosslinks between GroEL/ES and RNC1-510mut in the EL:ES2:RNC complex. Only crosslinks between GroEL/ES and L7/L12 (blue), GroEL and GroES (brown), and GroEL/ES and the NC (orange) are shown. b NC and ribosome stalk crosslink to the inner and outer surface of GroEL/ES, respectively. Crosslink sites are mapped onto the structures of ATP/BeFx-stabilised EL:ES2 (PDB:7VWX85), cut away to show the inner surface of the GroEL/ES cavity. Residues are separated according to whether they crosslink to L7/L12 (blue), the NC (orange), or connect GroEL and GroES (brown). c Visualisation of GroEL:ES2:RNC assemblies. Micrograph from nsEM of GroEL:ES2:RNC1-510mut complex. The scale bar corresponds to 100 nm. Examples of GroEL:ES2 complexes positioned near ribosomes (1-6), isolated double-capped EL:ES2 (7), or single-capped EL:ES1 (8) complexes are circled. 500 independent micrographs were collected. d NC density in the GroEL/ES cavity. Left: 3D reconstructions of GroEL:ES/RNC complexes from nsEM. Two classes can be distinguished, with additional density (black) in one or both chambers. The number of particles contributing to each reconstruction is given at the bottom. Right: The structure of GroEL/ES with encapsulated Rubisco (PDB: 7VWX85) docked into map 1. Source data are provided in Supplementary Data 3.
