Fig. 5: NicheNet predicts the mechanisms driving lymphatic remodeling.

a UMAP of 21 cell subsets, including LEC subsets and other cell types in LNs. b A schematic representation of the NicheNet analysis of intercellular communications that induce target genes in CD200⁺ HEY1⁺ LECs in metastatic LNs. CD200⁺ HEY1⁺ LECs were set as the receiver and the other cell types in LNs were set as the sender. c Predicted top ligands and their target genes in the CD200⁺ HEY1⁺ LECs. AUPR: area under the precision-recall curve. d Circos plot showing the links between predicted ligands from LN cells and their potential target genes in CD200⁺ HEY1⁺ LECs. Low-weight links were removed for clarity. e Relative expression of predicted targets in metastatic or distant LNs. f Potential receptors expressed by CD200⁺ HEY1⁺ LECs associated with each predicted ligand. Nine patients’ samples were integrated for this analysis (a–f).