Fig. 2: Fewer committed myogenic cells in E17.5 mdx muscles.
From: Intrinsic dysfunction in muscle stem cells lacking dystrophin begins during secondary myogenesis
a Representative immunofluorescence images of wild-type (WT) and mdx tibialis anterior (TA) and extensor digitorum longus (EDL) muscles from hindlimb transverse sections at embryonic day (E) 14.5 (top; scale bar, 50 µm) and magnified inset (bottom; scale bar, 25 µm). MYOG (green) denotes differentiating progenitor cells, and PAX7 (magenta) labels fetal MuSCs. b Immunofluorescence image examples of WT and mdx TA and EDL from hindlimb transverse sections at E17.5 (scale bar, 200 µm) and magnified inset (scale bar, 50 µm). MYOG (green) denotes differentiating progenitor cells, DAG1 (yellow) marks fetal sarcolemma, and PAX7 (magenta) labels fetal MuSCs. Enumeration of myogenic cells expressing either PAX7 or MYOG at E14.5 (c) and E17.5 (d) and normalized to mm2. e Proportion of MYOG+ cells relative to entire PAX7+ and MYOG+ cell population at E14.5. and E17.5. n = 7 per genotype at E14.5, and n = 12 per genotype at E17.5. Box plots show the median, the 25th and 75th percentiles, and whiskers are the minimum and maximum values. Data analyzed using two-way ANOVA corrected for multiple comparisons using Sidak’s post hoc test where *p < 0.05, **p < 0.01, and ***p < 0.001. Exact pvalues included in the Source Data.
