Fig. 4: Single cell RNA-sequencing uncovers reduced numbers of mdx progenitors.
From: Intrinsic dysfunction in muscle stem cells lacking dystrophin begins during secondary myogenesis
a Uniform manifold approximation and projection (UMAP) visualization of wild type (WT) and mdx myogenic cells isolated from Pax7-nGFP E17.5 fetal limbs for single cell RNA-sequencing libraries. 9000 cells are illustrated per genotype. b Bar plot illustrating library cluster proportions for WT and mdx myogenic libraries. n = 2 biological replicates. For a, b, clusters are schematically depicted in hierarchical order from fetal (f) MuSCs to myocytes and grouped by genotype. c Feature plots illustrating the expression of Pax7 and commitment markers Myog, Mymk and Acta1. d WT and mdx library proportions split by cell cycle phase, as determined by Seurat’s cell cycle scoring. e Proportion of library expressing proliferation markers (Cenpa, Mki67 or Cdk1), split by fMuSC and progenitor cell identity. f Gene ontology terms enriched in genes significantly downregulated in mdx compared to WT pseudobulked myogenic cell libraries, as identified by Seurat’s FindMarkers function with default Wilcoxon test. GO term enrichment was performed using enrichGO with default parameters, including the hypergeometric test and Benjamini-Hochberg correction for multiple testing. For d–f, n = 2 biological replicates. Exact pvalues are included in Supplementary Data 2–4. See also Supplementary Fig. 6.
