Fig. 7: Reduced NUMB expression and polarization in mdx fetal MuSCs.

a Violin plot of Hes1 and Numb expression in wild type (WT) and mdx scRNA-seq libraries derived from GFP⁺ cells isolated from Pax7-nGFP E17.5 limbs and split by cluster. b Feature plot of Hes1 expression in WT and mdx scRNA-seq libraries derived from GFP⁺ cells isolated from Pax7-nGFP E17.5 limbs and split by genotype. In a, b, n = 2 biological replicates. c Left: Representative immunofluorescence images of GFP⁺ (green) cells isolated from E17.5 WT and mdx:Pax7-nGFP limbs expressing NUMB (magenta) and counterstained with DAPI. Scale bar, 50 µm. Right: Example 3D projected images of a fMuSC expressing polarized and non-polarized NUMB expression. Scale bar, 2 µm. d Mean fluorescence intensity (MFI) of NUMB-Alexa Fluor (AF) 546 in GFP⁺ cells. e Proportion of GFP⁺ cells expressing NUMB. f Proportion of GFP⁺ NUMB⁺ cells expressing polarized NUMB. g Percent of entire GFP⁺ cells expressing polarized NUMB. Between 180 and 1600 cells assessed per replicate. n = 5 WT, n = 4 mdx. Statistical analysis performed using two-tailed unpaired ttests (** p < 0.01, ***p < 0.001, ns = not significant). Exact pvalues included in the Source Data. Box plots show the median, the 25th and 75th percentiles, and whiskers are the minimum and maximum values.