Fig. 8: Elevated type I IFN levels in m.5019 A>G mice following an in vivo LPS challenge. | Nature Communications

Fig. 8: Elevated type I IFN levels in m.5019 A>G mice following an in vivo LPS challenge.

From: An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice

Fig. 8

a Schematic of in vivo lipopolysaccharide (LPS) endotoxemia model experiment. b, c Volcano plot of Olink target T48 mouse cytokine and chemokine profiling of m.5019A>G mice vs wildtype (WT) mice serum injected intraperitoneally (i.p.) with phosphate-buffered saline (PBS) (n = 4; 2 h) or LPS (n = 10; 2 h). d Interferon-β (IFN-β) levels in serum of WT and m.5019A>G mice injected i.p. with PBS (n = 4; 2 h) or LPS (n = 10; 2 h). e Ifnb1 expression in kidney tissue of m.5019A>G mice vs WT mice serum injected i.p. with PBS (n = 4; 2 h) or LPS (n = 10; 2 h) (P = 0.0002). f Volcano plot of kidney tissue proteomic analysis of m.5019A>G mice vs WT mice injected i.p. with LPS (n = 10; 2 h). g Sepsis score of WT and m.5019A>G mice injected i.p. with PBS (n = 4; 2 h) or LPS (n = 10; 2 h) (P = 0.00001699). Data are log2FC or mean ± s.e.m. n number represents independent biological replicates (mice) from a minimum of two independent experiments. P-values calculated using multiple two-tailed unpaired t tests corrected for multiple comparisons using the Benjamini, Krieger and Yekutieli method or one-way ANOVA corrected for multiple comparisons using the Tukey method. a Created in BioRender. Dwane, L. (2025) https://BioRender.com/kk5tnv3.

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