Table 1 Fitting parameters for PET-FCS
BmrA NBD construct | a1 | τ1 (μs) | a2 | τ2 (μs) | a3 | τ3 (μs) |
|---|---|---|---|---|---|---|
S516C | 0.05 \(\pm\)0.01 | 8 \(\pm\)2 | -- | -- | -- | -- |
S516C-N459W | 0.19 \(\pm\)0.03 | 80 \(\pm\)30 | 0.62 \(\pm\)0.06 | 2.5 \(\pm\)0.4 | 0.72 \(\pm\)0.06 | 0.24 \(\pm\)0.04 |
S516C-N459W-W413F | 0.19 \(\pm\)0.01 | 39 \(\pm\)5 | -- | -- | 0.18 \(\pm\)0.01 | 0.57 \(\pm\)0.01 |
S516C-N459W + ADP | 0.35 \(\pm\)0.03 | 24 \(\pm\)4 | 0.56 \(\pm\)0.04 | 1.5 \(\pm\)0.2 | 0.42 \(\pm\)0.04 | 0.13 \(\pm\)0.03 |
S516C-N459W + ATP | 0.22 \(\pm\)0.08 | 253 \(\pm\)16 | 0.51 \(\pm\)0.06 | 7 \(\pm\)2 | 0.95 \(\pm\)0.06 | 0.30 \(\pm\)0.04 |
S516C-N459W-W413F + ADP | 0.18 \(\pm\)0.01 | 104 \(\pm\)12 | 0.18 \(\pm\)0.01 | 2.7 \(\pm\)0.2 | -- | -- |
S516C-N459W-W413F + ATP | 0.19 \(\pm\)0.02 | 177 \(\pm\)40 | 0.20 \(\pm\)0.01 | 5.7 \(\pm\)0.7 | -- | -- |
