Fig. 5: Label-free visualization of whole-trunk dynamics in 3D-PanoPACT.

a The steps to reconstruct dynamics of the whole trunk at 10 Hz using the spatiotemporal-integration (STINT) method. b The MIP and depth-encoded images from the frontal view of the whole trunk. RL right lung, LL left lung, MLL middle liver lobe, LMLL left middle liver lobe, LLLL left lateral liver lobe, MA mesenteric artery, SI small intestine, ST stomach, CE cecum, SV seminal vesicle, SC spermatic cord, TE testicle, PE penis, AC arteria cruralis. c The cross-sections from the frontal view of the whole-trunk image (orange arrows in b) at different time points, indicating that the imaging depth extends to a minimum of 20 mm. Each cross-section was obtained by averaging a 1 mm thick 3D image in the thickness direction. d The maximum intensity projection (MIP) and depth-encoded images from the backside view of the whole trunk. IBAT interscapular brown adipose tissue, SP spleen, LK left kidney, RK right kidney. e The cross-sections from the backside view of the whole-trunk image (cyan arrows in d) at different time points.