Fig. 1: FUS-ERG can form hollow co-condensates with dsDNA containing GGAA microsatellite sequence.
From: Deciphering the molecular mechanisms of FET fusion oncoprotein–DNA hollow co-condensates
a GFP-FUS-ERG can form biomolecular condensate in the concentration of 1, 2, 5, 10 and 20 μM. b 5 μM GFP-FUS-ERG mixed with 0.6 μM 25-bp random dsDNA (i), 0.6 μM 25 bp 2 × GGAA dsDNA (iii); 0.6 μM 25 bp 4 × GGAA dsDNA (iv). dsDNA was labeled with AlexaFluor647. (ii) and (v) Normalized intensity profiles in (i) and (iv). (c) (i) GFP-FUS-ERG mixed with 25 bp 4 × GGAA dsDNA; (ii) Phase diagram in (i). (d) (i) GFP-FUS-ERG (9YS) can form biomolecular condensate in the concentration of 1, 2, 5, 10, and 20 μM; (ii) 5 μM GFP-FUS-ERG (9YS) mixed with 0.6 μM 25-bp 4 × GGAA dsDNA. (iii) GFP-FUS-ERG (27YS) cannot form biomolecular condensate in 20 μM; (iv) 5 μM GFP-FUS-ERG (27YS) mixed with 0.6 μM 25-bp 4 × GGAA dsDNA. (e) (i) GFP-FUS-ERG (5RA) can form biomolecular condensate in the concentration of 1, 2, 5, 10, and 20 μM; (ii) 5 μM GFP-FUS-ERG (5RA) mixed with 0.6 μM 25 bp 4 × GGAA dsDNA. (iii) GFP-FUS-ERG (9RA) can form biomolecular condensate in the concentration of 5, 10, and 20 μM; (iv) 5 μM GFP-FUS-ERG (9RA) mixed with 0.6 μM 25-bp 4 × GGAA dsDNA. All in vitro droplet assays were executed under physiological conditions, specifically 40 mM Tris-HCl (pH 7.5), 150 mM KCl, 2 mM MgCl2, 1 mM DTT and 0.2 mg/mL BSA, with thorough mixing and a 30-minute incubation period prior to imaging, unless otherwise indicated. Scale bar: 5 μm in (a, b(i), (iii), (iv), c(i), d, and e). Scale bar: 2 μm in b(i) insert and b(iv) insert. Source data are provided as a Source Data file.
