Fig. 1: Neutralization pattern and establishing persistent fraction of SARS-CoV-2 strains in the neutralization assay. | Nature Communications

Fig. 1: Neutralization pattern and establishing persistent fraction of SARS-CoV-2 strains in the neutralization assay.

From: Spike conformational and glycan heterogeneity associated with furin cleavage causes incomplete neutralization of SARS-CoV-2

Fig. 1

A The neutralization pattern of COVA2-15, S309, and COVA2-17 against WT pseudovirus in HEK293T cells expressing ACE2 (blue), ACE2/TMPRSS2 (red), or ACE2/TMP/NRP1 (green) receptors. Data are presented as mean values +/− SD (B). The neutralization pattern of the authentic virus (WT) by COVA2-15, S309, and COVA2-17 in the same cells as in (A). C The neutralization pattern of various pseudoviruses against indicated antibodies, hACE2-Fc, and nanobody S1-49. The assays in A-C were done in two technical repeats. Data in A-C are presented as mean values +/− SD (D). The maximum neutralization values calculated from data in B for the indicated pseudoviruses and the standard deviation in maximum neutralization is based on three independent experiments. The epitope classes for receptor binding site (RBS) antibodies in RBD are indicated by sub-sites for those whose precise site is defined. CR is an acronym for complete resistance and indicated if virus is completely resistant to the given antibody. In such cases the persistent fraction is 100%. E. The percent residual infection of pseudovirus in the neutralization assay is plotted as a function of serum dilution. The blue line represents the median neutralization curve derived from all the sera (n = 22) tested, and the dotted line below the median neutralization indicates the saturation point of maximum reduction of infection. F The PF of infection of indicated pseudoviruses against the sera in E (n = 22) is shown as percent infection in the presence of the highest concentration of serum antibodies. The statistical difference was calculated using the Kruskal-Wallis test, and the multiple comparison between the pseudoviruses was based on Dunn’s post-test multiple comparison. Adjusted P-values < 0.0001 (****), < 0.0019 (**), and 0.02 (*) are indicated. All data is based on at least two independent repeats. All statistical analyzes were done on measurements done on distinct samples.

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