Fig. 5: Antibody specificities.
A Summary of Env epitopes targeted by polyclonal antibodies from each participant at 26 weeks as determined by EMPEM. Protomer designates that antibody-induced trimer dissociation into monomers was observed and does not imply that the antibodies are monomer-specific. For samples with an asterisk (*), additional Fab binding was observed in 2D classification but epitope mapping in 3D was unsuccessful. gp41-GH: gp41-glycan hole. gp41-base: gp41 trimer base. B Composite figures of the various antibody specificities detected by the 3D analysis are shown (side and top views), based on EMPEM data for PubID 202. C Binding ratio of wild type antigen (BG505 SOSIP.GT1.1) to mutant as determined by binding antibody multiplex assay (BAMA) (median fluorescence intensity (MFI): BG505 SOSIP.GT1.1 to BG505 SOSIPv8.1-GT1.1 apex-KO, BG505 SOSIPv4.1-GT1.1 CD4bs-KO and BG505 SOSIPv8-GT1.1 super-KO (apex and CD4bs epitope KO mutations combined); BG505 SOSIP.664 to ConM-BG505 V1V2 and BG505 SOSIP.v5 base-KO + 613 T. Serum samples from all 23 per-protocol participants were tested at each time point. Samples were tested in replicates and a mean of two replicates was reported. Wild type MFI at a 1:50 dilution was divided by mutant MFI at similar dilution for final calculations. Box plots indicate median values, 25th and 75th percentiles; whiskers represent minima and maxima. Source data are provided as a Source Data file.
