Fig. 4: Abasic Sites, not oxo⁸G, dominate aniline cleavage–dependent RNA oxidation signals.
From: Mapping of HOCl-oxidized RNA identifies abasic sites as major damage and oxidation product of oxo8G
a–c Comparative RNA sequencing profiles of an unmodified RNA 38mer (top panel), its oxo8G-congener (middle panel) and abasic-congener (bottom panel) under distinct fragmentation conditions including alkaline (a) and Mg2+ (b) treatment in addition to no fragmentation (c), respectively. The y-axis indicates NormGcounts and the x-axis indicates RNA positions. Data of biological replicates are presented as mean ± SD, n = 3 for unmodified and abasic 38mers and n = 6 for the oxo8G-modified 38mer. d, e Signal strength of 38mers mixed in different ratios analyzed by AAS (d, left panel) and OAbSeq (e, right panel) methods. The three axes indicate the proportion [%] of the oxo8G 38mer, the abasic 38mer and the respective signal strength in NormGcounts. The unmodified 38mer was added to complete the mixture to 100%. Due to the 5’ specific ligation enrichment step during the library preparation protocol (see Methods section), AAS scores, including NormGcounts, do not show a linear dependence on the RNA modification level. Thus, the signals obtained for mixtures of oligonucleotides may not be strictly additive. Data points of one replicate are shown. Source data are provided as a Source Data file.
