Fig. 5: Blockage of CXCR2 alleviated pathological CD8+ TSCM cell-induced HVA level reduction in brain. | Nature Communications

Fig. 5: Blockage of CXCR2 alleviated pathological CD8+ TSCM cell-induced HVA level reduction in brain.

From: Migration of CD8 + TSCM cells into intestine via PPBP–CXCR2 axis increases host stress susceptibility by inhibiting gut microbiome-derived homovanillic acid

Fig. 5: Blockage of CXCR2 alleviated pathological CD8+ TSCM cell-induced HVA level reduction in brain.

The enriched KEGG pathway of identified differential metabolites (P < 0.05 and VIP > 1) in microbiota (A) and brain tissue (B) of Rag1−/− recipient mice by MetaboAnalyst. C The VIP value of metabolites that actively engage in the metabolic pathways. D The level of HVA in microbiota of Rag1−/− recipient mice detected by metabolomics analysis. **P = 0.0035 versus the control CD8+ TSCM cells group, n = 6/group. The level of HVA in brain tissue (E) and blood (F) of Rag1−/− recipient mice that received CD8+ TSCM cells detected by ELISA. ***P < 0.0001 versus the control CD8+ TSCM cells group, ###P < 0.0001 versus the CSDS CD8+ TSCM cells group, n = 6/group. G The HVA level in human plasma samples detected by HPLC. *P = 0.0371 versus the pHCs group, n = 18/group. H The HVA level in human microbiota samples detected by metagenomic analysis. ***P < 0.0001 versus the pHCs group, n = 55/group. I Animal experiment in CSDS model (CON+Vehicle, CSDS+Vehicle, CSDS + HVA). Components of this figure were created using Servier Medical Art templates, which are licensed under a Creative Commons Attribution 3.0 Unported License; https://smart.servier.com. The depressive-like behaviors of recipient Rag1−/− mice were evaluated by the SPT (J), TST (K), and FST (L). CON+Vehicle (n = 6), CSDS+Vehicle (n = 8), CSDS + HVA (n = 8). ***P = 0.0002 (J), **P = 0.0022 (K), and **P = 0.0031 (L) versus the CON+Vehicle group. ### P = 0.0002 (J), #P = 0.0234 (K), and ##P = 0.0031 (L) versus the CSDS+Vehicle group. D Kruskal-Wallis test followed by two-sided Dunn’s post hoc multiple comparison test. E–F, J–L One-way ANOVA followed by two-sided Holm-Sidak post hoc multiple comparison test. G–H Mann–Whitney U test (two-sided). D–H, J–L Data were presented as mean ± SD. Source data are provided as a Source Data file. CON control, CSDS chronic social defeat stress, CD8+ TSCM cells stem cell-like memory CD8+ T cells, HVA homovanillic acid, pHCs psychiatrically healthy controls, MDD major depressive disorder, SPT sucrose preference test, TST tail suspension test, FST forced swim test.

Back to article page