Fig. 2: Fabrication of bioengineered tissues. | Nature Communications

Fig. 2: Fabrication of bioengineered tissues.

From: Reconstruction of the lymphatic system by transplantation of a centrifuge-based bioengineered lymphatic tissue

Fig. 2

a Fluorescence microscopic observation of suspended and centrifuged mMSC/GFP at different times after seeding (day 1) of > 3 independent experiments. Scale bars, 500 μm. b Typical images of hLEC+hMSC tissue, hLEC+mMSC tissue, and NIH3T3 (fibroblasts) fabricated by a centrifugal cell stacking technique (day 5). Scale bars, 5 mm. c Paraffin-section image of an hLEC+hMSC tissue (day 5) of > 3 independent experiments. Scale bars, 50 μm. d Immunofluorescence images of a lymphatic endothelial cell marker Prox-1 in mMSC tissues (by single seeding without centrifugation), hLEC/mMSC mixed tissue (by single seeding without centrifugation), hLEC+mMSC tissue (by centrifugal cell stacking technique), and hLEC+mMSC tissue (by centrifugal cell stacking technique) of > 3 independent experiments. Blue, DAPI; Red, Prox-1. Scale bars, 100 μm.

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