Fig. 8: Distribution of cells in lymphatic node-like structures. | Nature Communications

Fig. 8: Distribution of cells in lymphatic node-like structures.

From: Reconstruction of the lymphatic system by transplantation of a centrifuge-based bioengineered lymphatic tissue

Fig. 8

a Representative immunofluorescence staining for human- or mouse-reactive Prox-1 in a lymph node-like structure formed in hLEC+hMSC tissue-transplanted LD mice (day 21) of > 3 independent experiments. Blue, DAPI; Green, mouse-reactive Prox-1: Red, human-reactive Prox-1. Scale bars, 1 mm (upper panel) and 200 μm (lower panel). b Representative immunofluorescence staining for CD3 and B220 in a lymph node-like structure formed in hLEC+hMSC tissue-transplanted LD mice (day 21) of > 3 independent experiments. Blue, DAPI; Green, CD3: Red, B220. Scale bars, 1 mm (upper panel) and 200 μm (lower panel). c Representative immunofluorescence staining for CD11b and CD11c in a lymph node-like structure formed in hLEC+hMSC tissue-transplanted LD mice (day 21) of > 3 independent experiments. Blue, DAPI; Green, CD11b: Red, CD11c. Scale bars, 1 mm (upper panel) and 200 μm (lower panel). d Proportion of various immune cell populations in lymph nodes or lymph node-like structures of hLEC+hMSC tissue-transplanted LD mice (days 7, 14, and 21). CD3+ cells, CD19+ cells, CD11c+ cells, and F4/80+ cells were gated by SSC and FSC. Data represent the mean ± standard deviation from 3 independent experiments.

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