Fig. 3: Cell-type composition of each ST spot and correlation with APC and CEG signatures.

Using cell-type specific RNA expression profiles derived from single-cell RNA-sequencing data we were able to deconvolute each ST spot into its cell-type composition. a The accumulated cell-type fractions are shown for all spots (left, n = 19,854) and separated by status of aggressiveness (right, aggressive n = 12,481, non-aggressive n = 7373) of 32 samples (non-aggressive n = 12, aggressive n = 20) from 8 (non-aggressive n = 3, aggressive n = 5) PCa patients. b Spearman correlation coefficients calculated for cell-types and signature activity for all spots (top), spots from the aggressive disease group (middle) and spots from the non-aggressive group (bottom) visualized by color (red = positive, blue = negative correlation). c Scatterplots with linear regression of APC and CEG signature ssGSEA score vs. Club and luminal epithelial cells contents individually for spots from the aggressive (red, n = 12,481) and non-aggressive (blue, n = 7373) disease group. The R- and p values (Wald test with t-distribution, two-sided) for each linear regression are given inside the box of each plot.