Fig. 4: Chemokine receptor-ligand interaction in tissue from patients with aggressive PCa. | Nature Communications

Fig. 4: Chemokine receptor-ligand interaction in tissue from patients with aggressive PCa.

From: Spatial multi-omics identifies aggressive prostate cancer signatures highlighting pro-inflammatory chemokine activity in the tumor microenvironment

Fig. 4: Chemokine receptor-ligand interaction in tissue from patients with aggressive PCa.

a Potential receptor-ligand interactions of the CXC chemokines of the CEG signature with ACKR1 and DPP4 between different tissue types found by combining the CellPhoneDB results obtained from ST data of each of the samples from patients in the aggressive disease group (patients n = 5, samples n = 20, spots n = 12,481). The means of the average expression of the receptor-ligand pair are visualized with a color gradient and the p values (obtained by using CellPhoneDB’s one-sided permutation test [n = 10,000] combined using Fisher’s method, no multiple testing adjustment, chosen significance level p = 0.005) by the size of the circles. Receptor-ligand pairs are shown on the y-axis and interacting spot class pairs are given on the top and bottom x-axis. b Expression of the CXC chemokines of the CEG signature, the chemokine receptor ACKR1, and DPP4 in spots with UMI count >0 from aggressive disease patient tissue shown as box and whisker plots (box spans interquartile range (IQR), centerline indicates median, whisker extend 1.5 IQR from first and third quartile, observations beyond shown as individual points; number of spots: ISUP1 n = 127, 39, 27, 36, 59, 136, 544 [CXCL1, CXCL3, CXCL5, CXCL6, CXCL11, ACKR1, DPP4], ISUP2 n = 84, 28, 2, 28, 66, 159, 449, ISUP3 n = 5, 2, 0, 5, 21, 96, 177, ISUP4 n = 44, 12, 0, 22, 39, 200, 1077, ISUP5 n = 4, 2, 0, 7, 20, 95, 403, Lymphocytes n = 15, 2, 1, 1, 47, 94, 49, Non-cancer glands n = 701, 391, 266, 354, 388, 478, 1780, Stroma n = 224, 111, 94, 105, 62, 716, 778, Lymphocyte-enriched stroma n = 160, 65, 68, 104, 122, 474, 545). c Correlation of ACKR1, CXCR4, and DPP4 expression with cell type fractions per spot using ST data from all 32 samples (non-aggressive n = 12, aggressive n = 20) from 8 (non-aggressive n = 3, aggressive n = 5) PCa patients, colored according to Spearman correlation coefficient (blue = negative, red = positive correlation). Correlations for the CXC-chemokines are visualized in Supplementary Fig. 6.

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