Fig. 8: TRACE duplex assay testing using a portable device suitable for on-site use.
From: Thermally programmed one-pot CRISPR assay for on-site pandemic surveillance
a Photograph and (b) exploded schematic view of the internal architecture of this compact assay device, which employs a communication module for smartphone interaction and an external power bank to maximize operating time. The dimensions of the device are 82 mm × 50 mm × 85 mm (length × width × height). c, d Schematic of (c) the simplified optical system of this device that supports single-probe (FAM) detection from (c) dual detection ports to enable simultaneous analysis of two samples. (e) Workflow indicating the smartphone-based assay setup, and real-time monitoring and interpretation of the assay results. f Correlation of TRACE J2L ΔF₂ signals detected with a qPCR system and the TRACE assay device using clinical samples (detailed results shown in Supplementary Fig. 29). g Normalized TRACE duplex assay RNase P ΔF₁ and MPXV J2L ΔF₂ signal of 12 MPXV-positive and 11 MPXV-negative clinical samples. Source data are provided as a Source Data file.
