Fig. 5: Fluorescence equilibrium binding shows distinct vilazodone affinities in Na+, K+ and NMDG+.
From: Structural basis of vilazodone dual binding mode to the serotonin transporter
Vilazodone binding to SERT coupled with an NHS dye in 150 mM Na+ (black circles) K+ (brown triangles) or NMDG+ (cyan squares) buffer using spectral shift binding isotherm assay at a ratio between 670 and 650 nm (fraction bound). The derived KD values for vilazodone are 1.0 [0.4; 2.3], 7.1 [3.5; 14], and 13 [10;17] nM for Na+, K+, and NMDG+, respectively (means [S.E.M. interval]). Data are shown as biological repeats (for Na+ and K+ n = 3, for NMDG+ n = 4), performed in triplicate.
