Fig. 6: Vilazodone induces distinct conformational dynamics in Na⁺ and K⁺.

a, b Fluorescence emission spectra of purified SERT V86Anap (a) or F556Anap (b) excited at 360 nm. Spectra were recorded following incubation in 200 mM Na⁺ (blue) or K⁺ (red) with (dashed line) or without (solid line) 200 nM vilazodone. Color legends for (a) are the same as (b). Data were normalized to the fluorescence intensities at λmax in Na⁺ (V86Anap: 425 nm; F556Anap: 448 nm). Data are shown as mean ± S.E.M. (error envelopes) of n = 4 biological replicates, except from Na⁺, Na⁺ vilazodone and K⁺ performed in n = 5 replicates.