Fig. 7: Decarboxylative coupling of NHNI ester 3 with silyl enol ether 4 in live cells.
From: Biocompatible decarboxylative coupling enabled by N-hydroxynaphthalimide esters
a Reactions were performed in HUVEC cells with NHNI esters 3 (5 µmol) and silyl enol ether 4 (15 µmol) followed by blue LED irradiation at r.t. for 0, 5, 10, or 15 minutes. b UV-vis absorption spectrum of NHNI esters 3 (5 µmol), ketone 7 (5 µmol) and NI (5 µmol). c Representative confocal images of HUVEC cells treated with NHNI ester 3 and silyl enol ether 4. The cells were incubated with ester 3 and ether 4 for 30 min at 37 °C, then irradiated with blue LEDs for 0, 5, 10, or 15 min. Experiments were independently repeated three times with consistent results. Scale bar: 50 μm. NHNI ester N-hydroxynaphthalimide ester, NI naphthalimide, r.t. room temperature, UV–vis ultraviolet–visible, HUVEC human umbilical vein endothelial cell.
