Fig. 1: Hit discovery with SELs. | Nature Communications

Fig. 1: Hit discovery with SELs.

From: Barcode-free hit discovery from massive libraries enabled by automated small molecule structure annotation

Fig. 1: Hit discovery with SELs.

a SEL workflow: large libraries with diverse scaffolds are prepared via solid phase split and pool synthesis, with a wide compatibility range of chemical transformation. The libraries are then released from beads and screened in solution against immobilized targets. Binders are enriched from the rest of the library and analyzed via nanoLC-MS/MS. Hit compounds are identified and decoded by the custom-made COMET (COmbinatorial Mass Encoding decoding Tool) software. b Summary of SEL features, making it an excellent platform for rapid and straightforward early drug discovery.

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