Fig. 1: RNF20 levels are reduced in TOF patients and positively correlate with SpO2 levels.
a Dot plot showing the expression of RNF20 in different cell populations separated by Donor and Tetralogy of Fallot (TOF) patients from (GSE203274). Size of the dots reflects the percentage of cells expressing RNF20 and color represents the average expression level in the different cell types from donor and TOF patients. P-values were determined using the two-sided Wilcoxon test provided by FindMarkers. b Representative genes and enriched GO terms in marker genes for EC2. P-values were calculated using a hypergeometric test as performed by Metascape (v.3.5). c Spearman correlation between RNF20 expression and clinical traits in TOF patients (n = 25). d Dot plot showing the correlation between SpO2 levels and RNF20 gene expression in samples from TOF patients (n = 25). Each black dot represents an independent biological sample. The blue line corresponds to the linear regression fit, while the surrounding gray area denotes the 95% confidence interval, indicating the range within which the true regression line is likely to lie with 95% certainty. Spearman correlation was used to assess correlations between gene expression levels and clinical data in (c, d), and p-values were estimated using a two-tailed test based on a t-distribution approximation. e Relative mRNA expression of RNF20, GLUT1, PDK1 and LOX in HUVECs cultured in normoxia (n = 3) and hypoxia (1% oxygen level, 24 h, n = 3), determined by qPCR. Statistical analysis between two groups in (e) was performed using an unpaired two-tailed Student’s t test. Data are shown as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
