Fig. 1: Sexual stages, differential expression analysis and data integration of four diatom species.
From: Conserved genetic markers reveal widespread diatom sexual reproduction in the global ocean
a Drawings of the major cell stages during sexual reproduction in one centric and three pennate species. Stages for which RNA-sequencing data are included in our comparative analysis are indicated by encircled letters: (S) pheromone Signaling, (P) gametangia - Pre-gametic, (GZ) Gametes and Zygotes, (A) Auxospores. SIP sex-inducing pheromone, Ph-* and Ph+*: hypothetical sex pheromones of P. multistriata b Stacked bar plots showing the number of differentially expressed (DE) genes on a 5% false discovery rate level for each sexual cell stage of each species. “Overall” includes genes DE in at least one cell stage. Bars are colored to show the proportion of up- and down-regulated genes during sexual reproduction relative to the vegetative controls. c Heatmap showing cross-species and cross-stage expression conservation during sex. Upper triangle: number of shared homologous gene families, whose genes are DE in both sexual stages/species. Diagonal: number of gene families with DE genes in a given stage/species. Lower triangle: Pearson correlations between the average fold changes of significantly DE genes for each homologous gene family. Correlation coefficients are shown within each tile. GFs gene families. Log2FC log2(fold change).
