Fig. 1: First level clustering of snRNAseq from sciatic nerves from 2-3, 15-16, 20-30 months old mice. | Nature Communications

Fig. 1: First level clustering of snRNAseq from sciatic nerves from 2-3, 15-16, 20-30 months old mice.

From: Fibroblast growth factor signaling induces a chondrocyte-like state of peripheral nerve fibroblast during aging

Fig. 1

a Schematic diagram of the single nuclei isolation, FACS, and library preparation. Elements were created in BioRender. Preissl, S. (2025) https://BioRender.com/2nbvklt. b Heatmap of the 2 top differentially expressed genes in each of the 19 first level clusters: Endoneurial fibroblasts (Abca6, Apod), Perineurial fibroblasts (Ntng1, Gpc6), Epineurial cells (Opcml, Sema3c), Chondrocyte-like state cells (Ptch1, Itgbl1), Pericytes (Mgp, Gpx3), VSMC (Myh11, Dgkb), EC1 and 2 (Slco1a4, Kap), Nerve associated EC (Pecam1, Eln), Lymphatic EC (Mmrn1, Ccl21a), Adipose tissue 1 (Car3, Acsl1), Adipose tissue 2 (Nnat, Tenm4), Low-Plp1 mSC (Sorcs1, Ctnna3), Moto-neuron associated mSC (Pmp22, Slit2), High-Plp1 mSC (Hcn1, Mpz), nmSC (Csmd1, Cadm2), Macrophages (F13a1, Rbpj), B-cells (Ikzf3, Igkv1-110), T- and NK cells (Skap1, Gm2682). Cluster color code is maintained through the figure. n = 3, biological replicates. c, d UMAP embedding of 19,175 high-quality nuclei color-coded by cell type (c) or age (d) red box highlights adipocytes and green box highlights chondrocyte-like state fibroblasts. e Violin plots illustrating different signature genes expressed in 19 clusters. f Relative percentage of clusters across ages. Adipose tissue cells and chondrocyte-like state cells relative presence increases with age. Green boxes mark the chondrocyte-like state fibroblasts, and red boxes mark adipocyte clusters.

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