Table 3 Primary antibodies utilized in this study
Target Antigen | Host species | Provider |
|---|---|---|
Cluster of differentiation 45 (CD45) 1:50 | Goat | AF114 R&D systems, Minneapolis, MN, USA |
Cluster of differentiation 68 (CD68) 1:250 | Mouse | MCA1957 Biorad Hercules, CA, USA |
Chondroitin sulfate proteoglycan 4 (CSPG4) 1:200 | Rabbit | ab129051 Abcam, Cambridge, UK |
Fatty Acid Binding Protein 4 (FABP4) 1:100 | Rabbit | ab92501 Abcam, Cambridge, UK |
Fibroblast growth factor 2 (FGF2) 1:100 | Mouse | MA5-15276 Invitrogen, Waltham, MA USA |
Forkhead box protein C2 (FOXC2) 1:10 (Tissue), 1:30 (ICC) | Sheep | AF6989 Novus biologicals, Centennial, CO, USA |
Gamma H2A histone family member X (γH2AX) 1:400 | Rabbit | 9718 Cell Signaling Technology, Danvers MA, USA |
Glucose transporter 1/Solute carrier family 2 (GLUT1/SLC2A1) 1:200 | Rabbit | ab150299 Abcam, Cambridge, UK |
Granulin (GRN) 1:100 | Rabbit | ab187070 Abcam, Cambridge, UK |
Potassium Voltage-Gated Channel Subfamily C Member 2 (KCNC2) 1:100 | Rabbit | PA5-36072 Invitrogen, Waltham, MA, USA |
Mannose Receptor C-Type 1 (MRC1) 1:100 | Rabbit | ab64693 Abcam, Cambridge, UK |
SRY-Box transcription factor 10 (SOX10) 1:100 | Rabbit | ab180862 Abcam, Cambridge, UK |
S100 calcium-binding protein B (S100B) 1:100 | Rabbit | ab52642 Abcam, Cambridge, UK |
SRY-Box transcription factor 9 (SOX9) 1:250 | Rabbit | ab185966 Abcam, Cambridge, UK |
SRY-Box transcription factor 9 (SOX9) 1:50 | Goat | AF3075 R&D systems, Minneapolis, USA |
Transforming Growth Factor Beta Receptor 1 (TGBR1) 1:400 | Rabbit | 30117-1-AP Proteintech, Rosemont, IL, USA |
Tyrosine Hydroxylase (TH) 1:100 | Rabbit | ab137869 Abcam, Cambridge, UK |
Vimentin (VIMENTIN) 1:100 | Mouse | ab20346 Abcam, Cambridge, UK |