Table 3 Primary antibodies utilized in this study

From: Fibroblast growth factor signaling induces a chondrocyte-like state of peripheral nerve fibroblast during aging

Target Antigen

Host species

Provider

Cluster of differentiation 45 (CD45)

1:50

Goat

AF114

R&D systems, Minneapolis, MN, USA

Cluster of differentiation 68

(CD68)

1:250

Mouse

MCA1957

Biorad

Hercules, CA, USA

Chondroitin sulfate proteoglycan 4 (CSPG4)

1:200

Rabbit

ab129051

Abcam, Cambridge, UK

Fatty Acid Binding Protein 4

(FABP4)

1:100

Rabbit

ab92501

Abcam, Cambridge, UK

Fibroblast growth factor 2

(FGF2)

1:100

Mouse

MA5-15276

Invitrogen, Waltham, MA USA

Forkhead box protein C2

(FOXC2)

1:10 (Tissue), 1:30 (ICC)

Sheep

AF6989

Novus biologicals, Centennial, CO, USA

Gamma H2A histone family member X

(γH2AX)

1:400

Rabbit

9718

Cell Signaling Technology, Danvers MA, USA

Glucose transporter 1/Solute carrier family 2

(GLUT1/SLC2A1)

1:200

Rabbit

ab150299

Abcam, Cambridge, UK

Granulin

(GRN)

1:100

Rabbit

ab187070

Abcam, Cambridge, UK

Potassium Voltage-Gated Channel Subfamily C Member 2

(KCNC2)

1:100

Rabbit

PA5-36072

Invitrogen, Waltham, MA, USA

Mannose Receptor C-Type 1 (MRC1)

1:100

Rabbit

ab64693

Abcam, Cambridge, UK

SRY-Box transcription factor 10 (SOX10)

1:100

Rabbit

ab180862

Abcam, Cambridge, UK

S100 calcium-binding protein B

(S100B)

1:100

Rabbit

ab52642

Abcam, Cambridge, UK

SRY-Box transcription factor 9

(SOX9)

1:250

Rabbit

ab185966

Abcam, Cambridge, UK

SRY-Box transcription factor 9

(SOX9)

1:50

Goat

AF3075

R&D systems, Minneapolis, USA

Transforming Growth Factor Beta Receptor 1

(TGBR1)

1:400

Rabbit

30117-1-AP

Proteintech, Rosemont, IL, USA

Tyrosine Hydroxylase

(TH)

1:100

Rabbit

ab137869

Abcam, Cambridge, UK

Vimentin

(VIMENTIN)

1:100

Mouse

ab20346

Abcam, Cambridge, UK