Fig. 1: Combining the loss of 53BP1 with an inhibitor of DNA-PKcs causes a decrease in No Indel EJ. | Nature Communications

Fig. 1: Combining the loss of 53BP1 with an inhibitor of DNA-PKcs causes a decrease in No Indel EJ.

From: 53BP1-RIF1 and DNA-PKcs show distinct genetic interactions with diverse chromosomal break repair outcomes

Fig. 1

A Schematic of the EJ7-GFP reporter assay, which is chromosomally integrated in HEK293 cells and measures No Indel EJ (i.e., repair of blunt DSB distal ends of two Cas9 DSBs, which restores the critical Gly67 codon of GFP). Cells are transfected with Cas9 and two sgRNA expression vectors, and GFP frequencies are normalized to transfection efficiency with parallel GFP transfections. B Loss of 53BP1 magnifies the effect of DNA-PKcs inhibition (M3814) on No Indel EJ. Frequencies represent the mean ± SD. n = 6 biologically independent transfections, except Parental cells n = 9. Statistics with an unpaired t-test using Holm–Sidak correction. *P = 0.0203, ***P = 0.0002, ****P < 0.0001, n.s. = not significant. C Immunoblot analysis of 53BP1 for the cell lines and conditions shown in (B). Source data are provided as a Source data file.

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