Fig. 3: 53BP1 loss and DNA-PKcs disruption, alone and together, cause a similar shift in deletion patterns. | Nature Communications

Fig. 3: 53BP1 loss and DNA-PKcs disruption, alone and together, cause a similar shift in deletion patterns.

From: 53BP1-RIF1 and DNA-PKcs show distinct genetic interactions with diverse chromosomal break repair outcomes

Fig. 3

A Loss of 53BP1 causes an increase in −2 and −7 deletions, similar to inhibition of DNA-PKcs, and DNA-PKcs inhibition does not cause a further increase in 53BP1-KO cells. Shown are deletion sizes for the samples shown in Fig. 2C. Frequencies represent the mean ± SD. n = 3 biologically independent transfections. Statistics with an unpaired t-test using Holm–Sidak correction. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s. = not significant. § = deletion size was only found in 1 or 2 replicates, † = deletion size was not found in any of the replicates, and ‡ = deletion size was found in <2 Parental replicates. B Microhomology use varies with distinct deletions. Shown is the fraction of microhomology used for each deletion size and experimental condition shown in (Fig. 2C). n = 3 biologically independent transfections. Source data are provided as a Source data file.

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